暗纹东方线粒体DNA控制区结构和系统发育分析

被引：12

作者：

邵爱华

朱江

史全良

陈葵

机构：

[1] 苏州大学生命科学学院

来源：

中国水产科学 | 2007年 / 03期

关键词：

暗纹东方; mtDNA控制区; 系统关系;

D O I：

暂无

中图分类号：

S917.4 [水产动物学]; Q951 [动物演化与发展];

学科分类号：

摘要：

以暗纹东方(Takifugu fasciatus)肝脏线粒体DNA为模板,参照GenBank中红鳍东方(Takifugu rubripes)线粒体DNA序列设计合成特异引物进行PCR扩增,获得了暗纹东方线粒体DNA控制区基因(818 bp)及5′端上游的tR-NAPro基因(71 bp)的全序列。控制区碱基组成为T32.2%,C 19.1%,A35.6%,G13.2%。对照其他已报道的鱼类控制区结构,对暗纹东方控制区的结构进行了分析,识别了其终止序列区、中央保守区和保守序列区,找到了终止相关的序列TAS以及保守序列(CSB1,CSB2,CSB3)。CSB1、CSB2序列相对保守,TAS与其回文基序可形成稳定的茎环结构,成为H-链复制延伸时的终止识别位点。同时运用DNA分析软件对暗纹东方与GenBank中其他10多种鱼类的mtDNA控制区序列进行比对,并选取东方属的7种鱼类mtDNA控制区序列构建分子系统树。结果显示控制区基因较适于科鱼类中同属不同种的系统发育分析。

引用

页码：352 / 360

页数：9

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