外源多胺对低氧胁迫下黄瓜幼苗根系生长及H+-ATP酶和H+-焦磷酸酶活性的影响

被引:19
作者
汪天
李娟
郭世荣
高洪波
王素平
机构
[1] 南京农业大学园艺学院
关键词
多胺; 低氧; H+-ATP酶; H+-焦磷酸酶; 黄瓜幼苗;
D O I
暂无
中图分类号
S642.2 [黄瓜];
学科分类号
090202 ;
摘要
采用营养液水培方式,研究了根际低氧胁迫下外源多胺对黄瓜幼苗植株根系生长,内源多胺含量与质膜H+-ATP酶、液泡膜H+-ATP酶和焦磷酸酶活性的影响。结果表明,根际低氧胁迫显著抑制黄瓜幼苗根系的生长,外源Put(腐胺)和Spd(亚精胺)可缓解低氧胁迫对根系的生长抑制,多胺主要以Spd的形式发挥促进性的生理作用,Put通过转化为Spd发挥作用;低氧胁迫下黄瓜根系内源多胺含量略有提高,外源多胺处理可增加内源多胺的含量;低氧胁迫下外源Put和Spd处理后质膜H+-ATP酶活性显著提高,外源多胺对黄瓜根系液胞膜H+-ATP酶和H+-焦磷酸酶活性没有明显影响,说明低氧胁迫下外源多胺主要通过提高质膜H+-ATP酶活性而发挥生理作用。
引用
收藏
页码:637 / 642
页数:6
相关论文
共 11 条
[1]  
Differencesbetweentomatoandcucumberinethanol,lactateandmalatemetabolismsandcellsappHofrootsunderhypoxia. GuoSR,NadaK,KatohH,TachibanaS. JJpnSocHortSci . 1999
[2]  
Salttoleranceinsuspensionculturesofsugarbeet. BlumwaldE,PooleRJ. Plant and Physiology . 1987
[3]  
Polyamines,floralinductionandfloraldevelopmentofstrawberry. ElisabethT,Martin-TanguyJ. PlantGrowthRegul . 1995
[4]  
Further evidence that cytoplasmic acidosis is a determinant of flooding intolerance in plants. Roberts,JKM,Andrade,FH,Anderson,IC. Plant Physiology . 1985
[5]  
Bradford. Analytical Biochemistry . 1976
[6]  
RelationshipbetweenH+-ATPaseactivityandfluidityoftonoplastinbarleyrootsunderNaClstress. ZhangWH,ChenQ,LiuYL. ActaBotSin . 2002
[7]  
MolecularcloningofvacuolarH+-pyrophosphataseanditsdevelopmentalexpressioningrowinghypocotylsofmungbean. YoichiN,MasayoshiM. Plant and Physiology . 1998
[8]  
Similaritiesanddifferencesbetweenthetonoplast-typeandthemitochondrialH+-ATPaseofoatroots. WangYZ,SzeH. Journal of Biochemistry . 1985
[9]  
SuppressionofS-adenosylmethioninedecarboxylaseactivityisamajorcauseforhigh-temperatureinhibitionofpollengerminationandtubegrowthintomato. SongJJ,NadaK,TachibanaS. PlantCellPhysiol . 2002
[10]  
ImportanceofRhodospirillumrubrumH+-pyrophosphataseunderlow-energyconditions. García-ContrerasR,CelisH,RomeroI. Journal of Bacteriology . 2004