The number, weight, volume, density, shape and distribution of the root nodules were investigated in three different Casuarina equisetifolia plantations in Haikou, Hainan Province. The results showed that the biomass of root nodules in the sandy tidal soil was biggest, much bigger than that in sandy wind blown soil and sandy alluvial soil. The nitrogen content in soil was the most important factor that affected the biomass of the nodules. The other physical and chemical properties of the different soil types also affected the number, volume, shape and distribution of the nodules in soils.