大鼠局灶性脑梗死后细胞凋亡及热休克蛋白70表达的变化

被引：4

作者：

牛敬忠

王贤军

夏青

孙保亮

夏作理

机构：

[1] 泰山医学院附属医院神经内科,泰山医学院微循环重点实验室,北京大学药学院化学生物系,泰山医学院附属医院神经内科,泰山医学院微循环重点实验室山东泰安,山东泰安

来源：

中国微循环 | 2005年 / 03期

关键词：

热休克蛋白70; 细胞凋亡; 脑梗死;

D O I：

暂无

中图分类号：

R743.33 [脑栓塞];

学科分类号：

1002 ;

摘要：

目的探讨大鼠局灶性脑梗死后热休克蛋白70(HSP70)及细胞凋亡的变化。方法采用光化学法诱导制作大鼠局灶性脑梗死模型,冰冻切片行HSP70免疫组化染色,并用TdT-介导duTP-生物缺口末端标记(TUNEL)法检测凋亡细胞。结果免疫组化显示对照组和假手术组动物无HSP70免疫反应;局灶性脑梗死组12h即有HSP70的表达,48h时HSP70表达至高峰,阳性反应局限于半暗带。TUNEL结果显示,对照组和假手术动物无凋亡细胞;局灶性脑梗死组6h时在半暗区凋亡细胞出现,3d达高峰。结论局灶性脑梗死可诱导HSP70的表达、神经细胞凋亡,两者的分布一致,但HSP70的表达高峰明显早于细胞凋亡。

引用

页码：179 / 181

页数：3

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