脑缺血再灌注后脑内脑源性神经营养因子的基因表达与调节

被引：10

作者：

石向群

陆兵勋

米瑞发

范明

机构：

[1] 甘肃兰州市兰州军区总医院神经内科

[2] 广州南方医院神经内科

[3] 军事医学科学院三所

来源：

卒中与神经疾病 | 2000年 / 01期

关键词：

脑源性神经营养因子; 脑缺血再灌注; 海马; 原位核酸分子杂交;

D O I：

暂无

中图分类号：

R741.02 [神经病理学、病因学];

学科分类号：

0710 ; 071006 ;

摘要：

目的 :探讨脑缺血再灌注损伤后脑内脑源性神经营养因子 ( BDNF) m RNA水平的变化规律 ,推测BDNF对损伤病理的影响。方法 :线栓法制作大鼠脑缺血再灌注模型 ,原位杂交检测大鼠海马神经元内BDNFm RNA,图象分析间接定量 BDNFm RNA水平。结果 :( 1 )脑缺血及缺血再灌注均能诱导双侧海马神经元BDNFm RNA水平增高 ;( 2 )缺血损伤过重后海马神经元 BDNFm RNA水平增高的程度反而小 ;( 3 )再灌注后BDNFm RNA的水平继续升高 ,高峰值出现在 1 2小时 ,至 72小时降至对照组水平 ;两侧海马的变化趋势类似。结论 :海马神经元在脑缺血及再灌注损伤后将升高 BDNFm RNA的表达以保护神经元 ,海马各区 BDNFm RNA基础水平与海马神经元病理改变程度呈负相关。

引用

页码：1 / 3

页数：3

共 11 条

[1]

Spreading depression of activity in the cerebral cortex. Leao AAP. Journal of Neurophysiology . 1994

[2]

The role of brainderived neurotrophic factor in transient forebrain ischemia in rat brain. Ohara O,Yonekawa Y,Tanaka K,et al. Neurosurgery . 1994

[3]

Regulation of brain-derived neurotrophic factor gene expression after transient middle cerebral artery occlusion with and without brain damage. Kokaia Z,Zhou Q,Kokaia M,et al. Experimental Neurology . 1995

[4]

Selective induction of fibroblast growth factor receptor-1 mRNA after transient focal ischemia in the cerebral cortex of rat. Masumura M,Murayama N,Inoue T,et al. Neuroscience Letters . 1996

[5]

Neurotrophin induced second messenger responses in rat brain synaptosomes. Knipper M,Beck A,Rylett J,et al. NeuroReport . 1993

[6]

Localization of brainderived neurotrophic factor mRNA to neurons in the brain by in situ hybridization. Wetmore C,Ernfors P,Persson H,et al. Experimental Neurology . 1990

[7]

Coorrdinated expression of messenger RNAs for nerve growth focter, Brainderived neurotrophic factor and neurotrophin-3 in the rat hippocampas following transient forebrain ischemia. Takeda A,Onodera H,Suginoto A,et al. Neuroscience . 1993

[8]

Brain-derived neurotrophic factor is induced as an immediate early gene following N-methy1-D-aspartate receptor activation. Hughes P,Beilharz E,Gluckman P,et al. Neuroscience . 1993

[9]

Evidence for the role of N-methy1-D-aspartate ( NMDA) receptor in cortical spreading depression in the rat. Marranes R,Willems R,Prins E,et al. Brain Research . 1988

[10]

Regional brain-derived neurotrophic factor mRNA and protein levels followin transient forebrain ischemic in the rat. Kokaia Z,Nawa H,Uchino H,et al. Molecular Brain Research . 1996

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