PCR方法在区分牛奶牧场来源中的应用

被引:7
作者
徐仙 [1 ]
陈沁 [1 ]
雍克岚 [1 ]
韩奕奕 [2 ]
机构
[1] 上海大学生命科学学院
[2] 不详
关键词
PCR扩增; 牛奶; 随机引物;
D O I
10.13684/j.cnki.spkj.2008.12.019
中图分类号
TS252.7 [产品标准与检验];
学科分类号
摘要
以光明集团提供的采自8个牧场的牛奶为材料,提取DNA,利用PCR技术区分不同来源的牛奶是否有差异。PCR结果表明同一牧场不同天数的扩增条带基本一致;同一牧场不同引物的PCR扩增结果存在差异;同一引物不同牧场的PCR扩增结果有差异,主要表现在泳带数不同,强弱带的分子量也不一样,据此可以区分不同来源的牛奶。
引用
收藏
页码:258 / 261
页数:4
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