核因子κB反义寡核苷酸对肾小球硬化Caspase-3表达的影响

被引：4

作者：

李敏

纪泽泉

机构：

[1] 广州医学院第二附属医院儿科

来源：

实用儿科临床杂志 | 2007年 / 17期

基金：

广东省自然科学基金;

关键词：

肾小球硬化症,病灶性; 寡核苷酸类,反义; 核因子κB; Caspase-3;

D O I：

暂无

中图分类号：

R726.9 [小儿泌尿科学];

学科分类号：

100202 ;

摘要：

目的探讨核转录因子κB(NF-κB)反义寡核苷酸(ODN)对局灶性节段性肾小球硬化大鼠肾组织半胱氨酸天冬氨酸特异性蛋白酶(Caspase-3)、NF-κB表达影响。方法SD雄性大鼠分成3大组:假手术组(A组)、硬化组(B组)各6只,干预组(C组)9只;C组再分成3小组:正义ODN组(C1组)、无义ODN组(C2组)、反义ODN组(C3组)各3只。采用右肾切除加尾静脉注射多柔比星制成肾小球硬化模型。8周后各组分别予不同的药物干预,应用免疫组织化学检测肾组织Caspase-3、NF-κBp65表达。结果反义ODN干预d7,C3组大鼠尿蛋白、肾小球硬化指数、肾组织Caspase-3、NF-κBp65蛋白表达较B、C1、C2组明显减少,差异有统计学意义(Pa<0.05);C3组与A组比较,NF-κBp65蛋白表达无显著差异(P>0.05)。结论NF-κBODN能抑制肾小球NF-κB活性及Caspase-3表达,从而延缓肾小球硬化及细胞凋亡进程。

引用

页码：1302 / 1304

页数：3

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