间歇性张应力对小鼠单核/巨噬细胞RAW264.7分化成熟的影响

被引：6

作者：

郭大伟

张春艳

杨芳

于江波

卢恕来

郑照杰

彭斐

袁晓

宋玲

机构：

[1] 青岛市市立医院口腔科

来源：

中国口腔种植学杂志 | 2015年 / 20卷 / 04期

关键词：

张应力; RAW264.7; 破骨细胞; 细胞分化;

D O I：

暂无

中图分类号：

R783.6 [义齿学];

学科分类号：

100301 [口腔基础医学];

摘要：

目的:探讨张应力对破骨前体细胞小鼠单核/巨噬细胞株RAW264.7分化成熟的影响。方法:施加1000με、3000με、5000με间歇性张应力于体外诱导分化的RAW264.7细胞,采用抗酒石酸酸性磷酸酶(TRAP)染色和吖啶橙染色观察破骨细胞形成情况,并分析比较各组破骨细胞分化状况。结果:牵张应力促进了破骨细胞的增殖和分化,其TRAP阳性细胞增多,且随着牵张力量的增强TRAP阳性细胞数量呈增加趋势,但张应力增加到5000μεTRAP阳性细胞数量增加不明显。结论:间歇性的牵张力促进了小鼠单核/巨噬细胞RAW264.7向破骨细胞的分化。

引用

收藏

页码：151 / 154+148 +148

页数：5

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