Changes of c-fos and c-jun mRNA Expression in Angiotensin Ⅱ-induced Cardiomyocyte Hypertrophy and Effects of Sodium Tanshinone ⅡA Sulfonate

被引：15

作者：

周代星 ^{[1
]}

梁黔生 ^{[1
]}

何雪心 ^{[2
]}

占成业 ^{[1
]}

机构：

[1] Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

[2] School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology

来源：

Journal of Huazhong University of Science and Technology(Medical Sciences) | 2008年 / 05期

关键词：

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100103 [病原生物学];

摘要：

The changes of proto-oncogene c-fos and c-jun mRNA expression in angiotensin Ⅱ (AngⅡ)-induced hypertrophy and effects of sodium tanshinone ⅡA sulfonate (STS) in the primary culture of neonatal rat cardiomyocytes were investigated. Twelve neonatal clean grade Wistar rats were selected. The cardiomyocytes were isolated, cultured and divided according to different treatments in the medium. The cardiomyocyte size was determined by phase contrast microscope, and the rate of protein synthesis was measured by [3H]-Leucine incorporation. The c-fos and c-jun mRNA expression in cardiomyocytes was detected by reverse transcription polymerase chain reaction (RT-PCR). It was found after cardiomyocytes were treated with AngⅡ for 30 min, the c-fos and c-jun mRNA expression in cardiomyocytes was increased significantly (P<0.01). After treatment with AngⅡ for 24 h, the rate of protein synthesis in AngⅡ group was significantly increased as compared with control group (P<0.01). After treatment with AngⅡ for 7 days, the size of cardiomyocytes in AngⅡ group was increased obviously as compared with control group (P<0.05). After pretreatment with STS or Valsartan before AngⅡ treatment, both of them could inhibit the above effects of AngⅡ (P<0.05 or P<0.01). It was suggested that STS could ameliorate AngⅡ-induced cardiomyocyte hy- pertrophy by inhibiting c-fos and c-jun mRNA expression and reducing protein synthesis rate of cardiomyocytes.

引用

页码：531 / 534

页数：4

共 5 条

[1]

Calcium–calcineurin signaling in the regulation of cardiac hypertrophy.[J].Benjamin J. Wilkins;Jeffery D. Molkentin.Biochemical and Biophysical Research Communications.2004, 4

[2]

Signal Transduction Pathways of Angiotensin II-Induced c-fos Gene Expression in Cardiac Myocytes In Vitro Roles of Phospholipid-Derived Second Messengers.[J].Jun-ichi Sadoshima;Seigo Izumo.Circulation Research.1993, 3

[3]

MOLECULAR CHARACTERIZATION OF ANGIOTENSIN-II-INDUCED HYPERTROPHY OF CARDIAC MYOCYTES AND HYPERPLASIA OF CARDIAC FIBROBLASTS - CRITICAL ROLE OF THE AT(1) RECEPTOR SUBTYPE [J].