二甲双胍对多囊卵巢综合征大鼠的排卵及对卵巢中IL-6、TNF-α表达的影响

被引：5

作者：

王慧兰

徐霞

郑志群

机构：

[1] 福建医科大学附属协和医院妇产科

来源：

福建医科大学学报 | 2013年 / 47卷 / 05期

关键词：

多囊卵巢综合征; 二甲双胍; 肿瘤坏死因子-a; 白细胞介素-6; 排卵;

D O I：

暂无

中图分类号：

R711.75 [卵巢疾病];

学科分类号：

100211 [妇产科学];

摘要：

目的探讨二甲双胍对多囊卵巢综合征(PCOS)大鼠的促排卵作用及其对卵巢组织IL-6mRNA、TNF-αmRNA表达的影响。方法将32只雌性SD大鼠随机分为3组:空白对照组(A组,n=10),自由摄取食物和水31d;PCOS组(B组,n=12),来曲唑1mg/kg灌胃,21d后予以5mL/kg生理盐水灌胃10d;二甲双胍干预组(C组,n=10),来曲唑1mg/kg灌胃,21d后予以6%的二甲双胍5mL/kg灌胃10d。每日阴道涂片观察大鼠动情周期,末次灌胃24h后测定各组大鼠血清黄体生成素(LH)、雌二醇(E2)、睾酮(T)、胰岛素(INS)、空腹血糖水平,通过H-E染色观察卵巢形态,实时荧光定量PCR检测各组大鼠卵巢白细胞介素-6(IL-6)mRNA、肿瘤坏死因子-α(TNF-α)mRNA的相对表达。结果 C组较B组大鼠血清T、LH、INS明显降低(P<0.05),卵巢局部IL-6mRNA、TNF-αmRNA的表达下降(P<0.05),卵巢形态恢复正常。C组大鼠于用药的第6d全组恢复排卵,B组大鼠持续无排卵直至第10d,仅1只大鼠自发恢复排卵。结论二甲双胍能抑制卵巢局部IL-6mRNA、TNF-αmRNA的表达,有助于恢复卵巢形态,促进排卵,改善PCOS大鼠内分泌代谢紊乱。

引用

页码：288 / 292

页数：5

共 7 条

[1]

Status of clomiphene citrate and metformin for infertility in PCOS [J].

Misso, Marie L. ;

Teede, Helena J. ;

Hart, Roger ;

Wong, Jennifer ;

Rombauts, Luk ;

Melder, Angela M. ;

Norman, Robert J. ;

Costello, Michael F. .

TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2012, 23 (10) :533-543

[2]

Metformin Improves Pregnancy and Live-Birth Rates in Women with Polycystic Ovary Syndrome (PCOS): A Multicenter, Double-Blind, Placebo-Controlled Randomized Trial [J].

Morin-Papunen, Laure ;

Rantala, Anni S. ;

Unkila-Kallio, Leila ;

Tiitinen, Aila ;

Hippelainen, Maritta ;

Perheentupa, Antti ;

Tinkanen, Helena ;

Bloigu, Risto ;

Puukka, Katri ;

Ruokonen, Aimo ;

Tapanainen, Juha S. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2012, 97 (05) :1492-1500

[3]

Low-grade chronic inflammation in the peripheral blood and ovaries of women with polycystic ovarian syndrome [J].

Xiong, Yong-lao ;

Liang, Xiao-yan ;

Yang, Xing ;

Li, Yi ;

Wei, Li-na .

EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2011, 159 (01) :148-150

[4]

Granulosa cell survival and proliferation are altered in polycystic ovary syndrome [J].

Das, M. ;

Djahanbakhch, O. ;

Hacihanefioglu, B. ;

Saridogan, E. ;

Ikram, M. ;

Ghali, L. ;

Raveendran, M. ;

Storey, A. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2008, 93 (03) :881-887

[5]

Apoptosis and expression of protein TRAIL in granulosa cells of rats with polycystic ovarian syndrome.[J].Juan Zhang;Guijin Zhu;Xinrong Wang;Bei Xu;Linli Hu.Journal of Huazhong University of Science and Technology.2007, 3

[6]

Increased activation of nuclear factor κB triggers inflammation and insulin resistance in polycystic ovary syndrome [J].

González, F ;

Rote, NS ;

Minium, J ;

Kirwan, JP .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (04) :1508-1512

[7]

Letrozole-induced polycystic ovaries in the rat: A new model for cystic ovarian disease [J].

Kafali, H ;

Iriadam, M ;

Ozardali, I ;

Demir, N .

ARCHIVES OF MEDICAL RESEARCH, 2004, 35 (02) :103-108

← 1 →