转基因烟草中的海藻糖测定

被引：9

作者：

周坚

杨波

戴秀玉

机构：

[1] 中国科学院微生物研究所!北京

来源：

微生物学报 | 2001年 / 03期

关键词：

高效液相层析(HPLC); 蒸发光散射检测器(ELSD); 转基因烟草; 海藻糖;

D O I：

10.13343/j.cnki.wsxb.2001.03.021

中图分类号：

Q533 [二糖];

学科分类号：

摘要：

The \%E.coli\% trehase synthalose gene(\%otsA\%) was transferred into \%Nicotiana tabacum\% mediated by \%Agrobacterium\%, but the method for detecting low concentration of trehalose in transgenic plant was not available.The high performance liquid chromatograph(HPLC) with evaporative light\|scatting detector (ELSD) using water:methyl cyanide(1∶2\^6 v/v) as mobile phase was established in this work. An ODS column Zorbax RX\|SIL was employed. the trehalose detection limits of ELSD was 5mg/L. From the linear relationship between the logarithm of trehalose concentration and the logarithm of peak area, it was shown there was 14\^7μg\5(g FW)\+\{-1\} in transgenic plant. The data strongly confirmed that trehalose was responsible for the improved stress tolerance of the tobacco.

引用

页码：378 / 380

页数：3