分子生物学技术在食源性致病菌检测中的研究进展

被引:12
作者
唐廷廷 [1 ,2 ]
韩国全 [1 ]
王利娜 [3 ]
肖玲 [1 ]
王成程 [1 ]
文昕 [1 ]
机构
[1] 四川农业大学食品学院
[2] 重庆三峡职业学院农林科技系
[3] 四川省成都市食品药品检验研究院
关键词
分子生物学技术; 食源性致病菌; 检测方法; 食品安全;
D O I
10.19812/j.cnki.jfsq11-5956/ts.2016.09.013
中图分类号
TS207.4 [食品的微生物检验];
学科分类号
摘要
食源性致病菌是导致食源性疾病的重要爆发根源之一。分子生物学检测技术因其敏感性强、特异性高、快速简便、省时省力等优点,在食源性致病菌的鉴定与检测中扮演着重要角色。本文系统阐述了利用分子生物学技术检测食源性致病菌的方法,包括多重PCR、实时荧光定量PCR、环介导等温扩增技术、基因芯片、液相芯片和基因探针技术等,分析了目前国内外学者对于这些技术由单一检测向多重检测的突破,及实现快速、高通量检测食源性致病菌的应用研究成果,总结了各种检测技术的优缺点,旨在为未来更好地发展快速、高通量检测食源性致病菌的技术方法提供参考。
引用
收藏
页码:3497 / 3502
页数:6
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