烟草SOC1基因的克隆和表达分析

被引：5

作者：

石永春

杨永银

刘卫群

机构：

[1] 河南农业大学生命科学学院

来源：

中国烟草学报 | 2014年 / 20卷 / 02期

关键词：

烟草; SOC1; 转基因; 光合; 抗坏血酸;

D O I：

暂无

中图分类号：

S572 [烟草（菸草）];

学科分类号：

摘要：

为明确SOC1基因在叶片中的生理作用,克隆到烟草中SOC1基因的完整开放阅读框,全长806 bp。农杆菌介导转化烟草后,获得26棵转基因植株,其中23棵鉴定为阳性植株,转化率为88.46%。与野生型烟草K326相比,获得的SOC1过表达烟株开花提前,株高增加,叶片宽大。半定量RT-PCR结果显示,SOC1过表达不影响叶片中Rubisco大亚基、蔗糖磷酸合成酶和蔗糖合成酶基因的表达水平,但提高了抗坏血酸氧化酶的表达水平;提高了碳酸酐酶活性、蔗糖含量和还原性抗坏血酸含量,并降低了抗坏血酸过氧化物酶活性。表明SOC1可能通过影响氧化还原状态而提高碳酸酐酶活性和光合速率。

引用

页码：99 / 103

页数：5

共 6 条

[1] Redox Regulation of Carbonic Anhydrases via Thioredoxin in Chloroplast of the Marine Diatom Phaeodactylum tricornutum [J].

Kikutani, Sae ;

Tanaka, Rie ;

Yamazaki, Yukiko ;

Hara, Satoshi ;

Hisabori, Toru ;

Kroth, Peter G. ;

Matsuda, Yusuke .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (24) :20689-20700

[2] Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches [J].

Smykal, Petr ;

Gennen, Jerome ;

De Bodt, Stefanie ;

Ranganath, Venkatesh ;

Melzer, Siegbert .

PLANT MOLECULAR BIOLOGY, 2007, 65 (03) :233-242

[3]

Ascorbic acid oxidase is dynamically regulated by light and oxygen. A tool for oxygen management in plants?[J] . Mario C. De Tullio,Samantha Ciraci,Rosalia Liso,Oreste Arrigoni. Journal of Plant Physiology . 2005 (1)

[4] Over-expression of ascorbate oxidase in the apoplast of transgenic tobacco results in altered ascorbate and glutathione redox states and increased sensitivity to ozone [J].

Sanmartin, M ;

Drogoudi, PD ;

Lyons, T ;

Pateraki, I ;

Barnes, J ;

Kanellis, AK .

PLANTA, 2003, 216 (06) :918-928

[5]

Functional Diversity, Conservation, and Convergence in the Evolution of the α-, β-, and γ-Carbonic Anhydrase Gene Families[J] . David Hewett-Emmett,Richard E. Tashian. Molecular Phylogenetics and Evolution . 1996 (1)

[6]

Regulation of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Activity[J] . A R Portis. Annual Review of Plant Physiology and Plant Molecular Biology . 1992

← 1 →