TLR4信号缺失对MPTP帕金森小鼠模型黑质Beta-catenin信号的表达影响

被引：2

作者：

张璐璐 ^{[1
,2
]}

张巧俊 ^{[1
]}

胡婧 ^{[3
]}

张妮 ^{[1
]}

机构：

[1] 西安交通大学第二附属医院康复医学科

[2] 西安市第四医院神经内科

[3] 重庆市中医院急诊科

来源：

免疫学杂志 | 2016年 / 32卷 / 06期

关键词：

帕金森病; β-catenin; TLR4; MPTP;

D O I：

10.13431/j.cnki.immunol.j.20160097

中图分类号：

R742.5 [震颤麻痹综合征]; R-332 [医用实验动物学];

学科分类号：

1002 ; 1001 ;

摘要：

目的观察帕金森小鼠模型中TLR4通路对Beta-catenin信号的表达影响。方法 C57BL小鼠和TLR4敲除(TLR4knock out,TLR4 KO)小鼠各36只,分别注射生理盐水和1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)。采用RT-PCR、Westernblot检测4组之间的Beta-catenin的m RNA和蛋白表达变化,进一步采用免疫荧光的方法检测黑质区星形胶质细胞中Beta-catenin的共表达变化。结果 RT-PCR及Western blot结果显示,与生理盐水注射组小鼠相比,C57BL、TLR4KO小鼠在MPTP注射后,mRNA和蛋白表达明显升高(P<0.01);并且,与C57BL小鼠MPTP注射组相比,TLR4KO小鼠MPTP注射组Beta-catenin的mRNA和蛋白表达明显升高(P<0.01)。进一步的免疫荧光结果显示,与C57BL小鼠MPTP注射组相比,TLR4KO小鼠MPTP注射组星形胶质细胞(GFAP)中表达的beta-catenin明显升高。结论 TLR4通路在亚急性帕金森MPTP模型中,对黑质beta-catenin蛋白的表达起重要调控作用,Toll样信号与Beta-catenin信号这种互动关系可能在帕金森发病机制中起着重要的作用。

引用

页码：496 / 500

页数：5

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