人胃癌组织RNA的抽提及其c-H-ras原癌基因的表达

被引:1
作者
章有章
钱关祥
钱晓岚
王璐
于丽莉
陈诗书
王瑞年
机构
[1] 上海第二医科大学生物化学教研室
[2] 上海第二医科大学病理学教研室
关键词
c-H-rasRNA; 人原发性胃癌; 原癌基因表达;
D O I
暂无
中图分类号
学科分类号
摘要
用自制平板式不锈钢组织粉碎器。直接从手术切除的新鲜人原发性胃癌组织中用异硫氰酸胍-氯化铯超离心法抽提组织总RNA,再用Oligo(dT)纤维素柱分离得到mRNA,以经典的Northern印迹及分子杂交、放射自显影法观察到人原发性胃癌组织中c-H-ras原癌基因的表达强于或等于自身对照的相应正常胃粘膜组织。
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页码:304 / 308
页数:5
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