人肝脏组织亚细胞组分中含硒蛋白的分离与测定

被引：3

作者：

赵九江

陈春英

章佩群

柴之芳

机构：

[1] 中国科学院高能物理研究所核分析技术开放实验室,北京信箱核分析室,中国科学院高能物理研究所核分析技术开放实验室,北京信箱核分析室,中国科学院高能物理研究所核分析技术开放实验室,北京信箱核分析室,中国科学院高能物理研究所核分析技术开放实验室,北京信箱核分

来源：

中国生物化学与分子生物学报 | 2001年 / 06期

关键词：

human liver; subcellular fractions; selenium\|containing proteins; hydride generation\|atomic fluorescence spectrophotometry; electrophoresis;

D O I：

10.13865/j.cnki.cjbmb.2001.06.031

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

The distribution of selenium\|containing proteins in the subcellular fractions of human liver components was treated by sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) and hydride generation\|atomic fluorescence spectrophotometry (HG\|AFS). About 24 kinds of Se\|containing proteins or protein subunits were found in subcellular fractions. Their molecular weights were mostly ranged from 20 to 30 kD and from 50 to 80 kD. Among them, about 61 kD and 21 kD Se\|containing proteins existed in each fraction with higher concentration. The 54 kD Se\|containing protein existed in nuclei, mitochondria, microsome and cytosol. The specific subcellular distributions of Se\|containing proteins suggest that Se could play specific important biological roles in each organelle.

引用

页码：800 / 803

页数：4

共 8 条

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