微克水平蛋白质的染料结合比色法——应用于尿蛋白和脑脊液蛋白定量

被引:7
作者
赵善政 [1 ]
陈明伟 [1 ]
机构
[1] 上海第二医学院附属瑞金医院检验科
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暂无
中图分类号
R446 [实验室诊断];
学科分类号
100208 ;
摘要
本文报道一种简易快速的微量蛋白定量法,适用于临床实验室作尿蛋白和脑脊液蛋白定量。测得正常成人尿蛋白为3.94±3.66 mg/dl 或24小时尿蛋白总量为54.3±47.4 mg。脑脊液正常蛋白含量为30.5±8.8 mg/dl(或13~48)。对方法的重现性、准确性、试剂配方、对不同蛋白的显色差异、以及 CBG 与蛋白结合前后的吸收光谱进行了实验研究。
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