中国人IL-18结合蛋白cDNA的克隆和序列分析

被引：7

作者：

傅奕

赵惠仁

裴冬生

机构：

[1] 徐州医学院生物化学与分子生物学研究中心!江苏徐州

来源：

免疫学杂志 | 2000年 / 04期

关键词：

白细胞介素-18结合蛋白; RT-PCR; cDNA克隆; 序列分析;

D O I：

10.13431/j.cnki.immunol.j.20000080

中图分类号：

R392.11 [免疫分子生物学（免疫化学）];

学科分类号：

摘要：

目的克隆人白介素 - 18结合蛋白 (h IL- 18BP)的 c DNA,以研究其结构与功能的关系。方法从中国汉族人的胎盘组织中提取总 RNA,以 RT- PCR方法扩增出全长 5 85个 bp的 IL- 18BP c DNA,将其与表达载体 pc DNA3连接 ,转化大肠杆菌 DH5 α,建立了 h IL- 18BP的 c DNA克隆。结果序列分析表明 ,与国外文献报道的人 IL- 18BP的 c DNA序列完全一致。结论 h IL- 18BP已成功地得到克隆。

引用

页码：254 / 256

页数：3

共 9 条

[1] TORIGOE K,USHIO S,OKURA T,et al.Purification and characterization of the human interleukin-18 receptor. Journal of Biological Chemistry . 1997
[2] OKAMURA H,TSUTSUI H,KOMATSU T,et al.Cloning of a new cytokine that induces IFN- production by T cells. Nature . 1995
[3] NOVICK D,KIM SH,FANTUZZI G,et al.Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response. Immunity . 1999
[4] ROTHE H,JENKINS NA,COPELAND NG,et al.Active stage of autoimmune diabetes is associated with the expression of a novel cytokine, IGIF, which is located near Idd2. The Journal of Clinical Investigation . 1997
[5] USHIO S,NAMBA M,OKURA T,et al.Cloning of the cDNA for human IFN- -inducing factor , expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol . 1996
[6] TERESA LB,ELISABETH T,TIMOTHY AB,et al.Cloning of a novel receptor subunit , AcPL, required for interleukin-18 signaling. Journal of Biological Chemistry . 1998
[7] NOVICK D,DINARELLO CA,RUBINSTEIN M.Soluble IL-18 receptor in human urine: characterization and purification (abstract ). Cytokine . 1997
[8] AIZAWA Y,AKITA K,TANIAI M,et al.Cloning and expression of interleukin-18 binding protein. FEBS Letters . 1999
[9] KOHNO K,KURIMOTO M.Molecule of the month interleukin-18, a cytokine which resembles IL-1 structurally and IL-12 functionally but exerts its effect independently of both. Clinical Immunology and Immunopathology . 1998

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