PCR-DGGE方法分析原油储层微生物群落结构及种群多样性

被引：49

作者：

佘跃惠

张凡

向廷生

刘彬彬

赵立平

周玲革

舒肤昌

机构：

[1] 长江大学化学与环境工程学院

[2] 上海交通大学生命科学技术学院

来源：

生态学报 | 2005年 / 02期

关键词：

注水井; 油井; 变性梯度凝胶电泳(DGGE); 多样性; 微生物提高原油采收率(MEOR);

D O I：

暂无

中图分类号：

Q939.97 [工业微生物学];

学科分类号：

082203 ;

摘要：

使用基于 16 S r DNA的 PCR- DGGE(变性梯度凝胶电泳 )图谱分析结合条带割胶回收 DNA进行序列分析 ,对新疆克拉玛依油田一中区注水井 (12 # 9- 11)和与该注水井相应的两个采油井 (12 # 9- 9S、13# 11- 8)井口样品微生物群落的多样性进行了比较并鉴定了部分群落成员。 DGGE图谱聚类分析表明注水井与两油井微生物群落的相似性分别为 30 %和 2 0 % ,而两油井间微生物群落结构的相似性为 5 4 %。DGGE图谱中优势条带序列分析表明注水井样品和油井样品中的优势菌群为未培养的环境微生物 ,它们与数据库中 α、γ、δ、ε变形杆菌 (Proteobacteria)和拟杆菌 (Bacteroidetes)有很近的亲缘关系。 DGGE与分子克隆相结合的分子生物学方法在研究微生物提高原油采收率 (MEOR)机理 ,以及指导 MEOR在油田生产中的应用有着重要的意义

引用

页码：237 / 242

页数：6

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