胶质细胞源性神经营养因子体外对脊髓运动神经元的作用

被引：1

作者：

宋学琴

吴淑玉

李春岩

王丽琴

王晓娟

机构：

[1] 河北医科大学第二医院神经内科

[2] 河北医科大学第二医院神经内科河北石家庄

[3] 河北石家庄

来源：

细胞与分子免疫学杂志 | 2003年 / 05期

关键词：

胶质细胞源性神经营养因子; 脊髓运动神经元; 体外培养; 抗神经微丝单克隆抗体;

D O I：

10.13423/j.cnki.cjcmi.003206

中图分类号：

R741 [神经病学];

学科分类号：

1002 ;

摘要：

目的 :观察不同浓度的胶质细胞源性神经营养因子 (GDNF) ,对大鼠胚胎脊髓运动神经元生长活性的作用。方法 :取大鼠胚胎脊髓腹侧组织体外分离 ,进行原代细胞培养 ,应用抗神经微丝单克隆抗体 (mAb)SMI32进行运动神经元的免疫细胞化学染色 ,从细胞形态学及应用MTT比色法 ,研究GDNF对大鼠脊髓运动神经元的影响。结果 :GDNF能明显促进体外培养的大鼠脊髓运动神经元存活及突起的生长 (P <0 .0 5 ) ,且具有剂量依赖的趋势。结论 :不同浓度的GDNF对体外培养的大鼠胚胎脊髓运动神经元 ,有不同程度的促生长作用

引用

页码：490 / 492

页数：3

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