培育苹果转基因植株的研究

被引：14

作者：

刘庆忠

Hammerschlag

F.A.

机构：

[1] 山东省果树研究所!泰安

[2] 美国农业部Beltsville农业研究中心

来源：

落叶果树 | 2000年 / 01期

关键词：

苹果; GUS基因; 农杆菌; 转基因植株;

D O I：

10.13855/j.cnki.lygs.2000.01.002

中图分类号：

S661.103.4 [];

学科分类号：

摘要：

采用“皇家嘎拉”苹果（ＭａｌｕｓｄｏｍｅｓｔｉｃａＢｏｒｋｈ）白化茎段外植体，通过根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）ＥＨＡ１０５（ｐ３５ＳＧＵＳｉｎｔ）介导，证明了培养基中存在生长素可促进基因转化，转化效率比对照提高２倍以上。将外植体与ＥＨＡ１０１（ｐ３５ＳＧＵＳ）株系在含有生长素的培养基中共培养，获得了转基因植株。采用ＰＣＲ分析和ＳｏｕｔｈｅｒｎＢｌｏｔ核酸杂交，确定ＧＵＳ基因已整合到苹果植株的染色体上。组织化学染色确定了ＧＵＳ基因在植株体内的表达。

引用

页码：5 / 9

页数：5

共 10 条

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