铅锌矿区重金属污染对微生物数量及放线菌群落结构的影响

被引：80

作者：

李小林 ^{[1
]}

颜森 ^{[1
]}

张小平 ^{[1
]}

韦成健 ^{[2
]}

机构：

[1] 四川农业大学资源环境学院微生物系

[2] 西南交通大学环境科学与工程学院环境工程系

来源：

农业环境科学学报 | 2011年 / 30卷 / 03期

关键词：

铅锌矿区; 微生物数量; 放线菌; BOX-AIR; 16SrDNAPCR-RFLP;

D O I：

暂无

中图分类号：

X172 [环境微生物学];

学科分类号：

083001 [环境科学];

摘要：

采集四川省汉源县富泉乡万顺铅锌矿区5个不同重金属浓度的土壤样品,进行了微生物数量及放线菌多样性的研究。经分离、纯化得到43株不同的放线菌,然后对其进行BOXAIR-PCR和16SrDNAPCR-RFLP分析。结果表明,铅锌矿区重金属复合污染对土壤微生物数量有较大的影响,随着铅锌矿区重金属污染程度的加剧,土壤微生物的总数下降。相关性分析表明,重金属含量与细菌数量呈极显著负相关(P<0.01),与放线菌数量、真菌数量呈显著负相关(P<0.05)。供试菌株的16SrDNA用HaeⅢ、HinfⅠ和TaqⅠ酶切后具有32种遗传图谱类型。BOXAIR-PCR的聚类结果表明在86%的水平上,所有菌株分为10个遗传类型,结果基本与16SrDNAPCR-RFLP聚类差异不大。来源于高重金属的含量样品的菌株基本聚在一起,可能是重金属含量影响了放线菌的分布。同时,16SrDNA序列聚类分析结合系统发育树分析表明链霉菌属是汉源铅锌矿区主要的放线菌属并且具有遗传多样性。

引用

页码：468 / 475

页数：8

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