以猪IgG重链恒定区为抗原载体的抗口蹄疫病毒DNA疫苗的研制

被引：15

作者：

李光金

严维耀

徐泉兴

盛祖恬

郑兆鑫

机构：

[1] 复旦大学生命科学学院遗传工程国家重点实验室!上海

[2] 上海农业科学研究院兽医研究所!上海

[3] 浙江省农业科学研究院病毒研究所!杭州

来源：

生物工程学报 | 2001年 / 03期

关键词：

口蹄疫病毒; DNA疫苗; 免疫球蛋白G; 抗原;

D O I：

10.13345/j.cjb.2001.03.022

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

摘要：

The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragments of 141～160 and 21～40 peptide epitopes of a strain of type O FMDV was chemically synthesized and arranged into a tandem repeat 141～160 (20AA)\|21～40 (20AA)\|141～160 (20AA).This tandem sequence was fused to the 3′ end of the heavy chain constant region gene of swine immunoglobulin G and was then cloned into mammalian expression vector pCDM8 to form a recombinant plasmid pCDM8FZ3.After pCDM8FZ3 was inoculated intramuscularly into guinea pigs,it elicited a neutralizing antibody response and a specific spleen T cell proliferative response,and 66% of the vaccinated animals were protected from viral challenge.Our study indicated that the heavy chain constant region of swine IgG can act as the carrier protein for FMDV peptide epitopes,and pCDM8FZ3 is a potential DNA vaccine candidate to prevent FMDV infection.

引用

页码：322 / 324

页数：3

共 9 条

[1]

Protection against footand-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Bittle J L,Houghten R A,Alexander H et al. Nature . 1982

[2]

Antibody responses in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins. Chinsangaram J,Beard C,Mason P W et al. Journal of Virology . 1998

[3]

The promise of nucleic acid vaccines. Restifo N P,Ying H,Hwang L et al. Gene Therapy . 2000

[4]

Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule. Zaghouani H,Steinman R,Nonacs R et al. Science . 1993

[5]

ImprovedimmunogenicityofapeptideepitopeafterfusiontohepatitisBcoreprotein. ClarkeBE,NewtonSE,CarrollAR,etal. Nature . 1987

[6]

Bacterially expressed antigenic peptide from foot-and-mouth disease virus capsid elicits variable immunogogic responses in animals. Winther M D,Allen G,Bomford R H et al. The Journal of Immunology . 1986

[7]

Cloning cDNA of major antigenic of foot-and-mouth disease virus and expression in E.coli. Kupper H,Keller W,Kruz C et al. Nature . 1981

[8]

Gale C et al.Mouse protection test as a predictor of the protective capacity of synthetic foot-and-mouth disease vaccines. Mulcahy G,Pullen L A. Vaccine . 1991

[9]

A T cell epitope in VP1 of foot-andmouth disese virus is immunodominant for vaccinated cattle. Collen T,Dimarchi R,Doel T R. The Journal of Immunology . 1991

← 1 →