ZFN/TALEN技术与作物遗传改良

被引：2

作者：

李奇

安海龙

机构：

[1] 山东农业大学生命科学学院,作物生物学国家重点实验室

来源：

植物生理学报 | 2013年 / 49卷 / 07期

关键词：

ZFN; TALEN; 基因组编辑; 作物遗传改良; 生物安全性;

D O I：

10.13592/j.cnki.ppj.2013.07.006

中图分类号：

S188 [农业生物工程];

学科分类号：

071007 ; 0828 ; 0836 ; 090102 ;

摘要：

近年来,以锌指核酸酶(zinc-finger nucleases,ZFNs)和TALE核酸酶(transcription activator-like effector nucleases,TAL-ENs)为代表的基因组编辑技术开始出现并快速发展。这种技术把锌指或TALE(DNA识别结构域)与核酸酶融合,使之能够在特定的位点切开基因组DNA,随后利用基因组的修复/重组机制人工编辑基因组序列。利用ZFN/TALEN基因组编辑技术,可以实现基因敲除、片段置换、片段删除、定点插入等遗传操作。本文对ZFN/TALEN技术的工作原理和其在植物上的成功案例进行了综述,并对其在作物遗传改良研究中的应用前景进行了探讨。

引用

页码：626 / 636

页数：11

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