豆科三属八种植物的核型及rDNA定位研究

被引：12

作者：

刘博

陈成彬

李秀兰

齐力旺

韩素英

机构：

[1] 南开大学生命科学学院,南开大学生命科学学院,南开大学生命科学学院,中国林业科学院林业研究所细胞生物学实验室,中国林业科学院林业研究所细胞生物学实验室天津,天津,天津,北京,北京

来源：

云南植物研究 | 2005年 / 03期

关键词：

槐属; 刺槐属; 紫穗槐属; 核型分析; 45S rDNA; 荧光原位杂交;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

对豆科槐属的国槐(SophorajaponicaL.)、五叶槐(S.japonicaL.f.oligophyllaFranch.)、龙爪槐(S.japonicaL.f·pendulaLoud.)、黄金槐(S.xanthanthaC.Y.Ma.)(以上均为四倍体,2n=4x=28)、红花槐(S.rubrifloraTsoong.,2n=3x=21),刺槐属的刺槐(Robiniapseudoa-caciaL.,2n=2x=22)、毛洋槐(R.hispidaL.,2n=2x=30)和紫穗槐属的紫穗槐(AmorphafruticosaL.,2n=2x=40)核型进行了分析,并应用荧光原位杂交技术进行了45SrDNA的染色体定位。槐属4个四倍体种各具4个45SrDNA位点,位于两对染色体的着丝粒周围;红花槐,3个45SrDNA位点位于第5组染色体随体区域。刺槐,4个rDNA位点位于两对随体染色体端部;毛洋槐,8个rDNA位点,4个位于两对染色体的随体及次缢痕,另4个位于两对染色体着丝粒周围。紫穗槐,6个45SrDNA位点,分别位于3对染色体的着丝粒和随体。本文对rDNA作为染色体标记在核型分析中的应用及在基因组中的分布特点进行了讨论。

引用

页码：261 / 268

页数：8

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