绿肥还田对双季稻根际土壤产甲烷古菌群落结构的影响

被引:11
作者
赵晓萌 [1 ]
刘婧娜 [2 ]
易丽霞 [3 ]
朱波 [3 ]
代红翠 [2 ]
胡跃高 [2 ]
曾昭海 [2 ]
机构
[1] 北京农学院生物科学与工程学院
[2] 中国农业大学农学与生物技术学院
[3] 长江大学农学院
关键词
产甲烷古菌; 群落结构; 双季稻; 紫云英; 黑麦草;
D O I
暂无
中图分类号
S511.42 []; S154.3 [土壤微生物学];
学科分类号
0901 ; 071012 ; 0713 ;
摘要
利用PCR-DGGE技术及克隆文库构建方法研究,尿素、紫云英鲜草翻压还田、黑麦草鲜草翻压还田和不施氮肥4种处理对双季稻不同生育时期(早稻季:分蘖期,拔节期,成熟期;晚稻季:分蘖期,扬花期,成熟期)稻田根际土壤中产甲烷群落结构的影响。结果表明,双季稻不同取样时期和各处理中产甲烷古菌群落结构稳定且相似,早稻季和晚稻季的优势群落均为甲烷微菌目(Methanomicrobiales)、Rice Cluster I(RC-I)、甲烷鬃菌科(Methanosaetaceae)、甲烷杆菌属(Methanobacterium),但早稻季产甲烷古菌群落的Shannon-Weiner指数(H)和丰富度指数(R)整体低于晚稻季。紫云英和黑麦草鲜草翻压还田处理较尿素处理更为明显地提高了双季稻(一年)稻田根际土壤中产甲烷古菌群落的Shannon-Weiner指数和丰富度指数,但均暂未对产甲烷群落结构产生决定性影响。
引用
收藏
页码:698 / 707
页数:10
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