慢性肾衰竭大鼠股骨cbfa1表达与骨密度关系的研究

被引：4

作者：

白亚玲

徐金升

韩佩桐

张俊霞

张胜雷

崔立文

张慧然

机构：

[1] 河北医科大学第四医院肾内科

来源：

中国骨质疏松杂志 | 2014年 / 20卷 / 06期

关键词：

慢性肾衰竭; 慢性肾脏病矿物质和骨代谢紊乱; 核心结核因子-a1; 骨密度;

D O I：

暂无

中图分类号：

R692.5 [肾功能衰竭]; R580 [];

学科分类号：

100221 [泌尿外科学];

摘要：

目的探讨股骨核心结合因子-a1(cbfa1)对慢性肾衰竭大鼠股骨密度变化的影响。方法将12只大鼠随机分为空白对照组、慢性肾衰竭模型组。造模成功后测量各组大鼠股骨的整体骨密度(Whole-bone mineral density,W-BMD)、松质骨骨密度(Trabecular-bone mineral density,T-BMD)、皮质骨骨密度(Cortical-bone mineral density,C-BMD),留取血清测定钙、磷等生化参数,采用免疫组织化学和HE染色的方法分别检测大鼠股骨中Cbfa1蛋白的表达及松质骨和皮质骨的形态学改变。结果与空白对照组比较,慢性肾衰竭模型组W-BMD、C-BMD降低,T-BMD升高(P<0.001);股骨松质骨Cbfa1表达增多,皮质骨Cbfa1表达减少(P<0.001);相关分析显示W-BMD与皮质骨Cbfa1蛋白表达呈正相关(r=0.979,P<0.001),与松质骨Cbfa1蛋白表达呈负相关(r=-0.974,P<0.001),与血清钙浓度呈正相关(r=0.730,P<0.001),与血清磷浓度呈负相关(r=-0.652,P<0.001)。结论 Cbfa1是肾性骨病导致的骨密度改变的预测因素,可能是Cbfa1对骨形态的影响而导致骨密度的改变。

引用

页码：653 / 657+717 +717

页数：6

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