磷脂酶D对ParA1诱导的烟草的细胞死亡和过氧化氢及莨菪亭积累的影响

被引：3

作者：

魏芳芳

王蕾

梁元存

王玉军

机构：

[1] 山东农业大学植物保护学院

来源：

中国生物化学与分子生物学报 | 2010年 / 26卷 / 10期

关键词：

磷脂酶D; ParA1; 细胞死亡; 过氧化氢; 莨菪亭;

D O I：

10.13865/j.cnki.cjbmb.2010.10.008

中图分类号：

Q915 [古动物学];

学科分类号：

0709 ; 070903 ;

摘要：

利用药理学方法,研究了烟草寄生疫霉(Phytophthora parasitica)分泌的蛋白激发子ParA1诱导烟草悬浮细胞后,磷脂酶D对ParA1诱导的过敏细胞死亡和其它防卫反应的影响.用100nmol/LParA1处理烟草悬浮细胞后能够诱导细胞死亡、过氧化氢和莨菪亭的积累.磷脂酶D抑制剂正丁醇能够抑制ParA1诱导的这些防卫反应,仲丁醇所起的抑制作用比正丁醇小,正丁醇和仲丁醇产生的抑制效果具有浓度依赖效应.而叔丁醇不能抑制ParA1诱导的这些反应.结果表明,磷脂酶D参与了ParA1诱导烟草悬浮细胞的信号传导过程.

引用

页码：949 / 954

页数：6

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