无创性延迟肢体缺血预适应对脑缺血再灌注大鼠存活素和血管内皮生长因子表达的影响

被引：2

作者：

鲁慧 ^{[1
]}

王彬成 ^{[1
]}

崔宁宁 ^{[1
]}

张艳春 ^{[1
]}

杨兰 ^{[2
]}

机构：

[1] 沧州市中心医院神经内科

[2] 河北医科大学第二医院神经内科

来源：

东南大学学报(医学版) | 2018年 / 37卷 / 05期

关键词：

无创性延迟肢体缺血预适应; 脑缺血再灌注; 大鼠; 存活素; 血管内皮生长因子;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

摘要：

目的:探讨无创性延迟肢体缺血预适应对脑缺血再灌注大鼠存活素和血管内皮生长因子(VEGF)表达的影响。方法:根据随机数字表法将90只大鼠分为假手术组(sham组)、脑缺血再灌注损伤组(I/R组)、脑缺血再灌注损伤+无创性延迟肢体缺血预适应组(I/R+NDLIP组),每组30只。测定各组大鼠神经缺损症状和行为评分、脑梗死范围,采用免疫组化二步法测定各组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞,采用Western blotting法测定存活素和VEGF蛋白表达。结果:sham组大鼠未出现神经缺损症状,I/R+NDLIP组大鼠神经缺损症状和行为评分低于I/R组(P <0. 05)。sham组大鼠未见脑梗死区域,I/R+NDLIP组大鼠脑梗死范围明显小于I/R组(P <0. 05)。I/R组和I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞表达均高于sham组(P <0. 05),I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞表达均高于I/R组(P <0. 05)。I/R组和I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF蛋白相对表达量均高于sham组(P <0. 05),I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF蛋白相对表达量均高于I/R组(P <0. 05)。结论:无创性延迟肢体缺血预适应可降低脑缺血再灌注对神经的损伤,减小脑梗死范围,其机制可能与无创性延迟肢体缺血预适应可上调梗死灶周边皮质区存活素和VEGF的表达有关。

引用

页码：797 / 801

页数：5

共 19 条

[1] Novel vascular endothelial growth factor blocker improves cellular viability and reduces hypobaric hypoxia-induced vascular leakage and oedema in rat brain
Saraswat, Deepika
Nehra, Sarita
Chaudhary, Kamal
Prasad, Siva C. V. S.
[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2015, 42 (05) : 475 - 484
[2] Protective Effect of Intestinal Ischemic Preconditioning on Ischemia Reperfusion-Caused Lung Injury in Rats
Wang, Zhidong
Ji, Yuanyuan
Wang, Shufeng
Wang, Rong
Li, Zongfang
Kang, Anjing
Xu, Huili
Shi, Min
Zhao, MinXia
[J]. INFLAMMATION, 2015, 38 (01) : 424 - 432
[3] Role of Mesenchymal Stem Cells Transfected With Vascular Endothelial Growth Factor in Maintaining Renal Structure and Function in Rats with Unilateral Ureteral Obstruction. OZBEK E,ADAS G,OTUNCTEMUR A et al. Exp Clin Transplant . 2015
[4] Safety and Feasibility of Remote Limb Ischemic Preconditioning in Patients With Unilateral Middle Cerebral Artery Stenosis and Healthy Volunteers. Li S,Ma C,Shao G,et al. Cell Transplantation . 2015
[5] Interactions between osteopontin vascular endothelial growth factor:implications for cancer. Ramchandani D,Weber GF. Biochimica et Biophysica Acta . 2015
[6] Limb ischemic preconditioning attenuates cerebral ischemic injury in rat model. Wang W,Yu X,Mo X,Zhang H,Zhu D. Perfusion . 2014
[7] Study on influence of transient ischemic attack on subsequent cerebral infarction. Huang HL,Wang N,Zhou H,et al. European Review for Medical and Pharmacological Sciences . 2016
[8] Survivin, a key component of the Wnt/β?catenin signaling pathway, contributesto traumatic brain injury-induced adult neurogenesis in the mouse dentate gyrus[J] . Lin Zhang,Rong Yan,Qi Zhang,Haining Wang,Xiaokui Kang,Jia Li,Shuyuan Yang,Jianning Zhang,Zhenlin Liu,Xinyu Yang. &nbspInternational Journal of Molecular Medicine . 2013 (4)
[9] Clinical benefits of aortic cross-clamping versus limb remote ischemic preconditioning in coronary artery bypass grafting with cardiopulmonary bypass: a meta-analysis of randomized controlled trials[J] . Qi-Wen Deng,Zhi-Qiu Xia,Yu-Xin Qiu,Yan Wu,Jia-Xin Liu,Cai Li,Ke-Xuan Liu. &nbspJournal of Surgical Research . 2014
[10] Intestinal ischemic preconditioning ameliorates hepatic ischemia/reperfusion injury in rats: Role of heme oxygenase 1 in the second window of protection[J] . Shoichi Kageyama,Koichiro Hata,Hirokazu Tanaka,Hirofumi Hirao,Toyonari Kubota,Yusuke Okamura,Keiko Iwaisako,Yasutsugu Takada,Shinji Uemoto. &nbspLiver Transpl . 2015 (1)

← 1 2 →