肺纤维化大鼠肺组织细胞凋亡及Fas/FasL基因的表达附视频

被引:3
作者
黄新建
郑金旭
万兵
严玉兰
机构
[1] 江苏大学医学院
关键词
肺纤维化; 细胞凋亡; 基因表达;
D O I
10.13312/j.issn.1671-7783.2007.03.008
中图分类号
R563 [肺疾病];
学科分类号
摘要
目的:检测大鼠肺纤维化时肺组织细胞凋亡及Fas/FasL基因的表达,探讨细胞凋亡在肺纤维化过程中的作用。方法:通过博来霉素复制大鼠肺纤维化模型,同时设立正常对照组,采用TUNEL法、RT-PCR和Western Blot法检测肺组织细胞凋亡、Fas/FasL mRNA以及蛋白的表达。结果:肺纤维化模型复制成功;模型组肺组织细胞凋亡率、Fas/FasL mRNA和蛋白表达均显著高于对照组。结论:细胞凋亡及Fas/FasL基因表达的上调在博来霉素致大鼠肺纤维化发生、发展的过程中起了重要作用。
引用
收藏
页码:212 / 214+218 +218
页数:4
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