根癌农杆菌转化紫草的研究

被引:9
作者
祁建军
宋经元
雷和田
张荫磷
机构
[1] 中国医学科学院药用植物研究所中国协和医科大学,中国医学科学院药用植物研究所中国协和医科大学,中国医学科学院药用植物研究所中国协和医科大学,中国医学科学院药用植物研究所中国协和医科大学北京,北京,北京,北京
关键词
紫草; 根癌农杆菌; 冠瘿组织培养; 紫草素;
D O I
暂无
中图分类号
Q943.1 [植物细胞的离体培养];
学科分类号
071007 ; 090102 ;
摘要
Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.
引用
收藏
页码:656 / 658
页数:3
相关论文
共 2 条
[1]  
Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon[J] . Koichiro Shimomura,Hiroshi Sudo,Hitoshi Saga,Hiroshi Kamada.Plant Cell Reports . 1991 (6)
[2]  
Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon. III. Comparison of shikonin derivatives of cultured cells and ko-shikon[J] . Y. Fujita,Y. Maeda,C. Suga,T. Morimoto.Plant Cell Reports . 1983 (4)