中国O1群E1 Tor霍乱弧菌产毒株表型多态性研究

被引：2

作者：

赵璇

张力

李杰

阚飙

梁未丽

机构：

[1] 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室

[2] 天津市疾病预防控制中心

来源：

中华流行病学杂志 | 2014年 / 05期

关键词：

霍乱弧菌; 生物型; ctxB; rstR;

D O I：

暂无

中图分类号：

R378.3 [弧菌];

学科分类号：

100103 ; 100705 ;

摘要：

目的了解近50年中国不同地区分离的O1群E1 Tor型霍乱弧菌产毒株的生物表型特征变化。方法采用多粘菌素B敏感试验、第Ⅳ组霍乱噬菌体裂解试验、VP试验和溶血实验进行表型特征分析。结果生物型表型特征分析表明133株菌具有典型的E1 Tor生物型表型特征,其余251株菌呈现不典型的E1 Tor生物型表型特征;综合ctxB、rstR基因型和生物型表型特征分析,385株检测菌株中64株为典型E1 Tor生物型菌株,21株菌有典型的E1 Tor生物型表型特征但携带古典型ctxB基因,杂合型特征菌有280株,根据ctxB、rstR和表型特征的不同组合可再分为45个杂合型。结论中国O1群E1 Tor霍乱弧菌菌株呈现明显的表型多态性,传统的表型分型特征不能有效区分古典生物型和E1 Tor生物型菌株。

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