毛细支气管炎患儿免疫功能状态评价的意义

被引：21

作者：

刘雅丽 ^{[1
]}

杨锡强 ^{[2
]}

机构：

[1] 杭州市第一人民医院儿科

[2] 重庆医科大学儿童医院

来源：

实用儿科临床杂志 | 2006年 / 10期

关键词：

毛细支气管炎; 细胞因子; TH1细胞; TH2细胞;

D O I：

暂无

中图分类号：

R725.6 [小儿呼吸系及胸部疾病];

学科分类号：

100202 ;

摘要：

目的探讨毛细支气管炎(毛支)患儿的免疫功能状态的意义。方法采用酶联免疫吸附分析法(ELISA)和逆转录聚合酶链反应(RT-PCR)测定25例毛支患儿外周血单个核细胞体外产生IL-12、18、10、4,IFN-γ及血浆IgE的水平及膜表面蛋白分子淋巴细胞活化基因3(LAG-3)mRNA的表达。门诊体检的健康儿童14例作为正常对照组。结果毛支患儿急性期IL-12 (390．94±95．88)ng／L及II-18(146．23±55 81)ng／L水平明显低于正常对照组[(452．44±195．07)、(246．69±121．29)ng／L] (P<0．05),IL-10(302．40±100．04)ng／L、IL-4(30．52±17．65)ng／L及IgE(82．90±51．4)ng／L水平明显升高(P分别<0．05, 0．01,0．05),IFN-γ(103．25±82．15)ng／L水平虽低于正常对照组,但无显著差异(P>0．05)。IFN-γ/IL-4比率(3．6±0．9:1．0) 较对照组(9．7±2．1:1．0)降低约3倍(P<0．05)。TH1细胞表面标记LAG-3 mRNA的表达明显低(P<0．05)。结论毛支患儿存在TH1/TH2失衡、TH1功能下降、TH2功能占优势的状态,与APC的功能状态及其所分泌的上游调控细胞因子(IL-12、18、 10)的平衡紊乱有关。

引用

页码：584 / 586

页数：3

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