猪POU1F1基因第3内含子MspⅠ酶切片段多态特征及其与生长性能相关性的研究

A 2 100 bp segment of the third intron of the porcine POU1F1 gene was amplified by PCR approach. Two polymorphic fragments of 1 680 bp(C allele) and 850/830 bp(D allele) were observed. In this paper, we discussed the MspⅠ polymorphic restriction sites in F2 stirp-Changdufengjiang pigs[Landrace×(Duroc×Fengjiang)], and studied the effect on growth performance of allele gene at MspⅠ restriction site. The result showed that three genotypes at MspⅠ polymorphic restriction sites had significant characteristics of distribution in the herds of Changdufengjiang pigs and the growth performance was affected.The weights between DD genotype and other genotypes in different stages of days(expect birth weight) differed significantly(0.01<P<0.05) or differed very significantly(P<0.01),and the daily gain between DD genotype and other genotypes in different stage of days also differed significantly or differed very significantly. In a word, the allele gene D played an important role in the early and the late growth performance.