番荔枝内酯诱导白血病细胞凋亡的机理

被引：5

作者：

朱孝峰

谢冰芬

李志铭

冯公侃

曾益新

刘宗潮

机构：

[1] 中山医科大学肿瘤防治中心肿瘤研究所!广东广州

[2] 中山

来源：

药学学报 | 2001年 / 07期

关键词：

番荔枝内酯; 细胞凋亡; 白血病细胞HL-60;

D O I：

10.16438/j.0513-4870.2001.07.005

中图分类号：

R733.7 [白血病];

学科分类号：

100214 ;

摘要：

目的　研究番荔枝内酯 (squamocin)诱导白血病细胞凋亡的机理。方法　DNA凝胶电泳法、荧光染色等检测细胞凋亡。试剂盒检测caspase 3的活性。Westernblot法检测PARP和caspase 3的剪切片断和磷酸化的SAPK JNK量的变化。结果　Squamocin处理HL 6 0细胞后 ,导致染色质浓缩、片断化 ,DNA梯形条带出现 ,完整PARP的 116ku条带逐渐减少 ,而 85ku的片断逐渐增加 ,caspase 3酶的活性也增加。Caspase 3的特异性抑制剂DEVD CHO预处理HL 6 0细胞 ,可阻止squamocin诱导的DNA片断化、PARP的剪切和细胞死亡。Squamocin激活HL 6 0细胞中SAPK JNK。结论　Squamocin诱导HL 6 0细胞凋亡依赖caspase 3途径的激活 ,squamocin激活caspase 3可能与SAPK JNK的激活相关

引用

页码：498 / 501

页数：4

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