丹酚酸B镁盐对兔洗涤血小板聚集和5-HT释放反应的影响(英文)

被引：12

作者：

王唯

王逸平

孙伟康

徐亚明

宣利江

机构：

[1] 中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所上海中国,上海中国,上海中国,上海中国,上海中国

来源：

Acta Pharmacologica Sinica | 2000年 / 09期

关键词：

丹酚酸B镁盐; 血小板聚集; 血清素; 凝血酶; 花生四烯酸类; 血栓素B2; 钙;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的:研究丹酚酸B镁盐（magnesium lithospermateB）对血小板聚集和5-HT释放功能的影响及其作用机制.方法:比浊法评价兔洗涤血小板的聚集反应;荧光分光光度法和放射免疫法观察血小板的5-HT与TXA2的释放;用Fura 2荧光探针测定血小板胞内钙浓度变化.结果:丹酚酸B镁盐50-800mg/L对凝血酶或花生四烯酸诱导的血小板聚集反应有剂量依赖性的抑制作用,当体系无外Ca2+存在,丹酚酸B镁盐的抑制作用增强,IC50为102 mg/L.药物对血小板的5-HT释放也有剂量依赖性的抑制作用,但对AA激发的TXA2释放无影响.同时,丹酚酸B镁盐对凝血酶激发的胞内钙升高有明显的抑制作用,并降低静息血小板[Ca2+]ⅰ.结论:丹酚酸B镁盐对体外洗涤血小板的聚集和5-HT释放反应有明确的抑制作用,可能与影响血小板胞内Ca2+浓度的机制有关.

引用

页码：93 / 97

页数：5

共 13 条

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