肺细胞外基质水凝胶治疗大鼠放射性肺损伤的实验研究

被引：5

作者：

吴鹏飞 ^{[1
]}

施光清 ^{[2
]}

范贤明 ^{[1
]}

肖贞良 ^{[2
]}

周菁 ^{[1
]}

机构：

[1] 西南医科大学附属医院呼吸科

[2] 中国人民解放军西部战区总医院呼吸科

来源：

中国现代医学杂志 | 2019年 / 29卷 / 12期

关键词：

肺损伤; 放射性; 大鼠,Sprague-Dawley; 气管内注射/注射; 肺ECM/水凝胶;

D O I：

暂无

中图分类号：

R730.55 [放射疗法];

学科分类号：

100234 [放射肿瘤学];

摘要：

目的探讨气管内注射肺细胞外基质(ECM)水凝胶对大鼠放射性肺损伤的疗效。方法脱细胞法制备肺ECM水凝胶,单次全肺20 Gy照射复制放射性肺损伤模型。实验一:照射后30 min将24只大鼠随机分为4组,分别注射肺ECM水凝胶0、300、500及800μl,比较注射后30 min的死亡率、动脉血氧分压(PaO2),免疫荧光染色观察水凝胶分布。实验二:将18只大鼠分为对照组(正常大鼠)、照射组(照射+气管内注射生理盐水)及ECM组(照射+气管内注射肺ECM水凝胶),注射时间为照射后30 min,剂量500μl,照射7 d后行肺病理学检查,ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果气管内注射肺ECM水凝胶300或500μl不会影响大鼠PaO2(P>0.05),而800μl则会影响大鼠PaO2(P <0.05),300、500及800μl组均能在肺泡表面观察到绿色荧光,其中800μl分布最均匀;照射组及ECM组大鼠血清中TNF-α、IL-6水平较对照组升高(P <0.05),ECM组大鼠血清中TNF-α、IL-6水平较照射组降低(P <0.05)。结论肺ECM水凝胶能减轻放射性肺损伤早期的炎症反应,可作为一种新的治疗策略。

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页码：1 / 6

页数：6

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