胶体金免疫层析法检测食用肉中的氨基脲

被引：3

作者：

向军俭

王五洲

唐勇

杨红宇

机构：

[1] 暨南大学抗体工程研究中心

来源：

食品科学 | 2009年 / 30卷 / 20期

基金：

广东省科技计划;

关键词：

氨基脲; 胶体金免疫层析(ICA); 试纸条;

D O I：

暂无

中图分类号：

R155.5 [食品卫生与检验];

学科分类号：

100403 ;

摘要：

制备特异性胶体金免疫层析试纸条,检测肉类食品中氨基脲的残留。用活性酯法将氨基脲(SEM)衍生物CPSEM交联到牛血清白蛋白(BSA)上制备完全抗原CPSEM-BSA,将硫酸铵沉淀法纯化的抗CPSEM单克隆抗体标记到柠檬酸三钠还原法制备的胶体金,通过优化C/T线及金标抗体工作浓度,制备检测氨基脲的胶体金免疫层析试纸条,并对试纸条的灵敏度、特异性、准确性和稳定性进行测定。制备的试纸条检测5min后即可用肉眼观察到结果;对SEM的最低检测限达0.72ng/ml;除与呋喃西林有弱交叉反应外,与其他同类物均无交叉反应;该试纸条对猪肉中添加的SEM检测结果与酶联免疫吸附测定法(ELISA)检测结果呈现了很好的相关性;试纸条在室温下保存7周后不会失效。通过制备针对SEM的试纸条所建立的胶体金免疫层析法(ICA),快速简便、灵敏度高、特异性强、准确性和稳定性好,基本能达到商用检测要求。

引用

页码：291 / 295

页数：5

共 9 条

[1]

细胞和分子免疫学实验技术[M]. - 第四军医大学出版社 , 金伯泉主编, 2002

[2] Enzyme immunoassay for semicarbazide - The nitrofuran metabolite and food contaminant [J].

Cooper, Kevin M. ;

Samsonova, Jeanne V. ;

Plumpton, Laura ;

Elliott, Christopher T. ;

Kennedy, D. Glenn .

ANALYTICA CHIMICA ACTA, 2007, 592 (01) :64-71

[3] Preparation of monoclonal antibodies against a derivative of semicarbazide as a metabolic target of nitrofurazone [J].

Gao, Aizhong ;

Chen, Qiaolin ;

Cheng, Yu ;

Lei, Jing ;

Zeng, Lingwen .

ANALYTICA CHIMICA ACTA, 2007, 592 (01) :58-63

[4]

Semicarbazide: occurrence in food products and state-of-the-art in analytical methods used for its determination[J] . Maria Beatriz de la Calle,Elke Anklam.Analytical and Bioanalytical Chemistry . 2005 (4)

[5]

Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography–tandem mass spectrometry[J] . Journal of Chromatography A . 2001 (1)

[6]

Human health perspective of environmental exposure to hydrazines: A review[J] . Gangadhar Choudhary,Hugh Hansen.Chemosphere . 1998 (5)

[7]

Determination of nitrofuran residues in avian eggs by liquid chromatography–UV photodiode array detection and confirmation by liquid chromatography–ionspray mass spectrometry[J] . Rosa Draisci,Luigi Giannetti,Luca Lucentini,Luca Palleschi,Gianfranco Brambilla,Luigi Serpe,Pasquale Gallo.Journal of Chromatography A . 1997 (1)

[8]

Development of an immunochromatographic assay for rapid detection of1-aminohydantoin in urine specimens .2 XU Y P,LIU L Q,LI Q S et al. Biomedical Chromatography . 2008

[9]

The place of gold in rapid tests .2 Chandler J,Gurmin T,Robinson N. IVD Technology . 2000

← 1 →