大黄酸对白细胞介素-6诱导的血管平滑肌细胞增殖的影响

被引：8

作者：

吕敏 ^{[1
]}

周媛媛 ^{[2
]}

马向华 ^{[3
]}

沈捷 ^{[3
]}

机构：

[1] 南京市建邺医院内科

[2] 南京市中医院

[3] 南京医科大学第一附属医院

来源：

现代生物医学进展 | 2009年 / 9卷 / 16期

关键词：

大黄酸; 白介素-6; 动脉粥样硬化;

D O I：

10.13241/j.cnki.pmb.2009.16.022

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:研究大黄酸对白细胞介素-6(IL-6)刺激的血管平滑肌细胞(VSMCs)增殖效应的影响。方法:(1)采用原代细胞培养方法建立大鼠VSMCs细胞模型,形态学倒置相差显微镜和透射电镜观察的方法进行细胞鉴定;(2)以四甲基偶氮唑盐(MTT)比色法观察不同浓度和不同时间的IL-6对VSMCs增殖能力的影响,以及不同浓度的大黄酸作用12小时对IL-6效应的干预作用。结果:IL-6能显著促进VSMCs增殖,促增殖效应在48h末达峰值且IL-6浓度在0～50.0ng/ml之间呈剂量依赖关系,差异具有统计学意义(P<0.05);而大黄酸可抑制IL-6诱导的VSMCs的增殖效应(P<0.01)。结论:IL-6可刺激VSMCs增殖,而大黄酸能够抑制IL-6的促增殖作用,有望用于干预动脉粥样硬化的发生发展,其机制可能与线粒体依赖的凋亡机制有关。

引用

页码：3075 / 3077+3080 +3080

页数：4

共 10 条

[1]

Invited review: Vascular cells contribute to atherosclerosis by cytokine- and innate-immunity-related inflammatory mechanisms[J] . Harald Loppnow. &nbspInnate Immunity . 2008 (2)

[2]

Rhein Inhibits TNF-[alpha]-Induced Human Aortic Smooth Muscle Cell Proliferation via Mitochondrial-Dependent Apoptosis[J] . Heo, Sook-kyoung,Yun, Hyun-jeong,Park, Won-hwan,Park, Sun-dong. &nbspJournal of Vascular Research . 2009 (4)

[3]

中药药理学[M]. 中国中医药出版社 , 侯家玉主编, 2002

[4]

C-Reactive Protein, Interleukin-6, and Soluble Adhesion Molecules as Predictors of Progressive Peripheral Atherosclerosis in the General Population: Edinburgh Artery Study[J] . Ioanna Tzoulaki,Gordon D. Murray,Amanda J. Lee,Ann Rumley,Gordon D.O. Lowe,F Gerald R. Fowkes. &nbspCirculation . 2005 (7)

[5]

SjoholmA,NystromT.Endothelialinflammationininsulinresist ance. The Lancet . 2005

[6]

Dehghan A,Hoek MV,Sijbrands EJ.Risk of Type2Diabe-tes Attributable to C-Reactive Protein and Other Risk Fac-tors. Diabetes Care . 2007

[7]

H. Loppnow,K. Werdan,M. Buerke.Vascular cells contribute to atherosclerosis by cytokine- and innateimmunity-related inflammatory mechanisms. Innate Immun . 2008

[8]

Liu S,Tinker L,Song Y,et al.A prospective study of in-flammatory cytokines and diabetes mellitus in a multiethnic cohort of postmenopausal women. Archives of Internal Medicine . 2007

[9]

Tzoulaki I,Murray GD,Lee AJ,et al.C-reactive protein, interleukin-6, and soluble adhesion molecules as predictors of progressive peripheral atherosclerosis in the general population: Edinburgh Artery Study. Circulation . 2005

[10]

Leinonen E,Hurt-Camejo E,Wiklund O,et al.Insulin resistance and adiposity correlate with acute-phase reaction and soluble cell adhesion molecules in type 2 diabetes. Atherosclerosis . 2003

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