藏波罗花根中两个酚苷的分离纯化、结构鉴定和抗氧化活性研究(英文)

被引：8

作者：

潘为高 ^{[1
]}

蒋思萍 ^{[2
]}

罗彭 ^{[1
]}

高平 ^{[3
]}

陈彬 ^{[2
]}

卜海涛 ^{[2
]}

机构：

[1] 广西中医学院

[2] 西藏高原生物研究所

[3] 四川大学生命科学学院生物资源与生态环境教育部重点实验室

来源：

药学学报 | 2011年 / 04期

关键词：

藏波罗花; 生物活性跟踪法; 抗氧化剂;

D O I：

暂无

中图分类号：

R284.1 [化学分析与鉴定];

学科分类号：

100804 [中药化学];

摘要：

利用生物活性跟踪法,通过硅胶吸附柱色谱、反相C18柱色谱和反相制备型HPLC,从藏波罗花的根中筛选到两个化合物。通过ESI-MS、GC-MS、1D-和2D-NMR进行结构鉴定,确定为acteoside(1)和isoacteoside(2)。1和2的-OH清除活性与苯甲酸接近,1和2的-OH及O-2-清除活性均远远高于维生素C,1和2的肝组织LPO抑制活性也高于BHT或维生素C,1和2在保护红细胞免受氧化损伤的活性也高于维生素C。在0.015 6至0.500 0 mg·mL-1浓度范围,1和2的-OH清除活性与浓度成正相关关系。在0.001 9至0.250 0 mg·mL-1浓度范围,随着浓度的增大,1和2的肝组织LPO抑制活性不断增强;但在0.250 0至1.000 0 mg·mL-1浓度范围,随着浓度的增大,LPO抑制活性反而有轻微的下降。

引用

页码：422 / 427

页数：6

共 8 条

[1]

Isolation; purification and structure identification of antioxidant compound from the roots of Incarvillea younghusbandii Sprague and its life span prolonging effect in Drosophila melanogaster[J] Weigao Pan;Siping Jiang;Peng Luo;Jun Wu;Ping Gao Natural Product Research 2008,

[2]

Phenylethanoid oligoglycosides and acylated oligosugars with vasorelaxant activity from Cistanche tubulosa[J] Masayuki Yoshikawa;Hisashi Matsuda;Toshio Morikawa;Haihui Xie;Seikou Nakamura;Osamu Muraoka Bioorganic & Medicinal Chemistry 2006,

[3]

Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity[J] Kyung Ah Koo;Seung Hyun Kim;Tae Hwan Oh;Young Choong Kim Life Sciences 2006,

[4]

Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes[J] Hsin-Ling Yang;Ssu-Ching Chen;Nai-Wen Chang;Jia-Ming Chang;Mei-Ling Lee;Pei-Chuan Tsai;Han-Hsuan Fu;Wei-Wan Kao;Hsiao-Chi Chiang;Hsuan-Hui Wang;You-Cheng Hseu Food and Chemical Toxicology 2006,

[5]

The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells[J] Jin Hee Lee;Ji Yun Lee;Hyo Suk Kang;Chan Hun Jeong;Hee Moon;Wan Kyunn Whang;Chang Jong Kim;Sang Soo Sim Archives of Pharmacal Research 2006,

[6]

Antioxidant property of an active component purified from the leaves of paraquat-tolerant Rehmannia glutinosa[J] Kim;Son;Chun;Kim;Chung;Hwang;Lee Redox Report 2005,

[7]

Isolation; structure elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes[J] R.W Owen;R Haubner;W Mier;A Giacosa;W.E Hull;B Spiegelhalder;H Bartsch Food and Chemical Toxicology 2003,

[8]

Composition for preventing andtreating degenerative cranial nerve disease with phenylethanoidderivative Kim HJ;Koo GA;Sung SH; KR;2004064830 2004,

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