水稻谷氨酰胺合成酶同工酶免疫学性质比较研究(英文)

被引：11

作者：

林清华

李常健

张楚富

彭进

PENGShao-Bing

JohnBENNETT

机构：

[1] 武汉大学生命科学学院!武汉,武汉大学生命科学学院!武汉,武汉大学生命科学学院!武汉,武汉大学生命科学学院!武汉,InternationalRiceResearchInstitute!POBox,MakatiCity,Philippines,InternationalRiceResearchInstitute!POBox,Ma

来源：

植物学报 | 2000年 / 05期

关键词：

抗原; 抗体; 谷氨酰胺合成酶; 同工酶; 水稻;

D O I：

暂无

中图分类号：

Q946.5 [酶];

学科分类号：

摘要：

用纯化的水稻 (OryzasativaL .)根部存在的两种谷氨酰胺合成酶 (GS)同工酶GSra和GSrb分别免疫兔子 ,得到相应的抗体。免疫扩散和免疫印迹实验表明 ,GSra或GSrb的抗体对GS及其同工酶是专一的。免疫沉淀试验表明 ,GSra或GSrb不仅识别它的相应的抗原 ,而且也能很好地识别彼此的抗原。这两种抗体也能较好地识别水稻叶片胞液型的GS1,但对水稻叶片和菠菜 (SpinaciaoleraceaMill.)叶片叶绿体型的GS2的识别较弱。这些结果表明 ,水稻根的GSra和GSrb有很相似的抗原性 ,他们是非常相似的蛋白质。揭示GSra和GSrb像GS1一样都是胞液型GS。

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页码：471 / 475

页数：5

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