利用ISSR DNA标记鉴定主要银杏栽培品种

被引:49
作者
沈永宝
施季森
赵洪亮
机构
[1] 南京林业大学国家林业局南方林木种子检验中心
[2] 江苏省邳州市林业局
关键词
银杏; ISSR标记; 鉴定;
D O I
暂无
中图分类号
S664.3 [银杏(白果)];
学科分类号
摘要
Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.
引用
收藏
页码:202 / 204
页数:3
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