实时定量PCR及其在轮状病毒检测中的应用

被引：12

作者：

王云瑾 ^{[1
]}

周旭 ^{[2
]}

余黎 ^{[1
]}

机构：

[1] 兰州生物制品研究所

[2] 上海生物制品研究所

来源：

微生物学免疫学进展 | 2012年 / 40卷 / 01期

关键词：

实时定量PCR; 探针; 轮状病毒; 应用; 综述;

D O I：

10.13309/j.cnki.pmi.2012.01.017

中图分类号：

R450 [];

学科分类号：

摘要：

实时定量PCR(Real-time polymerase chain reaction/quantitative Real-time polymerase chain reaction,Real-time PCR/qPCR)就是在PCR扩增过程中,通过荧光信号对PCR进程进行实时监测。它具有特异性强、灵敏度高、定量准确和快速等优点,在生物医学领域中得到广泛的应用。对实时定量PCR技术的原理和类型,实时定量PCR技术在生物医学领域的应用,尤其在轮状病毒诊断、检测及疫苗研究中的应用及其未来前景进行了综述。

引用

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页数：7

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