白头翁汤对溃疡性结肠炎模型小鼠肠道菌群及短链脂肪酸的影响

被引：44

作者：

胡静怡

朱磊

连紫宇

程成

冯皖

沈洪

机构：

[1] 南京中医药大学附属医院

来源：

南京中医药大学学报 | 2021年 / 37卷 / 06期

关键词：

白头翁汤; 溃疡性结肠炎; 肠道菌群; 短链脂肪酸;

D O I：

10.14148/j.issn.1672-0482.2021.0817

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的探究白头翁汤对溃疡性结肠炎(Ulcerative colitis, UC)模型小鼠的治疗作用及可能机制。方法采用葡聚糖硫酸钠(Dextran sulphate sodium, DSS)诱导UC模型小鼠,给予白头翁汤干预。实验过程中,每日记录小鼠体质量、腹泻、便血情况。实验结束后测量小鼠结肠长度,HE染色观察结肠病理变化,qPCR检测结肠炎症因子表达,16S rRNA检测小鼠肠道菌群组成,GC-MS法检测回盲瓣内容物短链脂肪酸(SCFAs)含量。结果与正常组小鼠相比,模型组小鼠体质量下降,结肠缩短(P<0.01),结肠组织中炎性细胞浸润,结构紊乱,部分形态消失;结肠组织中促炎因子IL-1β、IL-6和TNF-α表达增加(P<0.05,P<0.01)。肠道菌群结构失衡,厚壁菌门与拟杆菌门比值降低,回盲瓣内容物中丁酸、戊酸含量降低(P<0.01)。与模型组小鼠相比,白头翁汤干预后,小鼠体质量明显增加,结肠长度增加(P<0.05,P<0.01),结肠存在炎性细胞浸润,但结构清晰,IL-1β、IL-6和TNF-α表达下降(P<0.05,P<0.01),厚壁菌门与拟杆菌门比值增加,回盲瓣中丁酸、戊酸含量增加(P<0.05,P<0.01)。结论白头翁汤可能通过调节肠道菌群,改善SCFAs代谢起到治疗溃疡性结肠炎的作用。

引用

页码：817 / 822

页数：6

共 11 条

[1]

Baitouweng decoction alleviates dextran sulfate sodium-induced ulcerative colitis by regulating intestinal microbiota and the IL-6/STAT3 signaling pathway.[J].C.H.E.N. Xuan-qing;L.V. Xiang-yu;L.I.U. Shi-jia.Journal of Ethnopharmacology.2021,

[2]

From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites [J].

Koh, Ara ;

De Vadder, Filipe ;

Kovatcheva-Datchary, Petia ;

Backhed, Fredrik .

CELL, 2016, 165 (06) :1332-1345

[3]

Metabolite-sensing receptors GPR43 and GPR109A facilitate dietary fibre-induced gut homeostasis through regulation of the inflammasome [J].

Macia, Laurence ;

Tan, Jian ;

Vieira, Angelica T. ;

Leach, Katie ;

Stanley, Dragana ;

Luong, Suzanne ;

Maruya, Mikako ;

McKenzie, Craig Ian ;

Hijikata, Atsushi ;

Wong, Connie ;

Binge, Lauren ;

Thorburn, Alison N. ;

Chevalier, Nina ;

Ang, Caroline ;

Marino, Eliana ;

Robert, Remy ;

Offermanns, Stefan ;

Teixeira, Mauro M. ;

Moore, Robert J. ;

Flavell, Richard A. ;

Fagarasan, Sidonia ;

Mackay, Charles R. .

NATURE COMMUNICATIONS, 2015, 6

[4]

The gut microbiota, bacterial metabolites and colorectal cancer [J].

Louis, Petra ;

Hold, Georgina L. ;

Flint, Harry J. .

NATURE REVIEWS MICROBIOLOGY, 2014, 12 (10) :661-672

[5]

A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis [J].

Machiels, Kathleen ;

Joossens, Marie ;

Sabino, Joao ;

De Preter, Vicky ;

Arijs, Ingrid ;

Eeckhaut, Venessa ;

Ballet, Vera ;

Claes, Karolien ;

Van Immerseel, Filip ;

Verbeke, Kristin ;

Ferrante, Marc ;

Verhaegen, Jan ;

Rutgeerts, Paul ;

Vermeire, Severine .

GUT, 2014, 63 (08) :1275-1283

[6]

Activation of Gpr109a, Receptor for Niacin and the Commensal Metabolite Butyrate, Suppresses Colonic Inflammation and Carcinogenesis [J].

Singh, Nagendra ;

Gurav, Ashish ;

Sivaprakasam, Sathish ;

Brady, Evan ;

Padia, Ravi ;

Shi, Huidong ;

Thangaraju, Muthusamy ;

Prasad, Puttur D. ;

Manicassamy, Santhakumar ;

Munn, David H. ;

Lee, Jeffrey R. ;

Offermanns, Stefan ;

Ganapathy, Vadivel .

IMMUNITY, 2014, 40 (01) :128-139

[7]

Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells [J].

Furusawa, Yukihiro ;

Obata, Yuuki ;

Fukuda, Shinji ;

Endo, Takaho A. ;

Nakato, Gaku ;

Takahashi, Daisuke ;

Nakanishi, Yumiko ;

Uetake, Chikako ;

Kato, Keiko ;

Kato, Tamotsu ;

Takahashi, Masumi ;

Fukuda, Noriko N. ;

Murakami, Shinnosuke ;

Miyauchi, Eiji ;

Hino, Shingo ;

Atarashi, Koji ;

Onawa, Satoshi ;

Fujimura, Yumiko ;

Lockett, Trevor ;

Clarke, Julie M. ;

Topping, David L. ;

Tomita, Masaru ;

Hori, Shohei ;

Ohara, Osamu ;

Morita, Tatsuya ;

Koseki, Haruhiko ;

Kikuchi, Jun ;

Honda, Kenya ;

Hase, Koji ;

Ohno, Hiroshi .

NATURE, 2013, 504 (7480) :446-+

[8]

Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease [J].

Takaishi, Hirornasa ;

Matsuki, Takahiro ;

Nakazawa, Atsushi ;

Takada, Toshihiko ;

Kado, Shoichi ;

Asahara, Takashi ;

Kamada, Nobuhiko ;

Sakuraba, Atsushi ;

Yajima, Tornoharu ;

Higuchi, Hajime ;

Nagamu, Inoue ;

Ogata, Haruhiko ;

Iwao, Yasushi ;

Nomoto, Koji ;

Tanaka, Ryuichiro ;

Hibi, Toshifumi .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 298 (5-6) :463-472

[9]

Whole-genome transcription profiling reveals genes up-regulated by growth on fucose in the human gut bacterium "Roseburia inulinivorans" [J].

Scott, Karen P. ;

Martin, Jennifer C. ;

Campbell, Gillian ;

Mayer, Claus-Dieter ;

Flint, Harry J. .

JOURNAL OF BACTERIOLOGY, 2006, 188 (12) :4340-4349

[10]

Restricted distribution of the butyrate kinase pathway among butyrate-producing bacteria from the human colon [J].

Louis, P ;

Duncan, SH ;

McCrae, SI ;

Millar, J ;

Jackson, MS ;

Flint, HJ .

JOURNAL OF BACTERIOLOGY, 2004, 186 (07) :2099-2106

← 1 2 →