芥菜型油菜4-二氢黄酮醇还原酶基因的克隆和表达分析

被引:13
作者
严明理
刘显军
刘忠松
官春云
袁谋志
熊兴华
机构
[1] 湖南农业大学油料作物研究所
关键词
芥菜型油菜; 4-二氢黄酮醇还原酶基因; 克隆; RT-PCR分析;
D O I
暂无
中图分类号
S565.4 [油菜籽(芸薹)];
学科分类号
摘要
利用同源克隆方法,在芥菜型油菜中克隆了DFR基因。在DNA和cDNA中扩增的DFR基因大小分别为1612bp和1214bp。该基因含有5个内含子,开放阅读框为1158bp,预计编码385个氨基酸,预测分子量为42886.0Da,推测的等电点为5.54。DFR基因在芥菜型油菜紫叶芥和黑籽近等基因系的叶片、胚和种皮中都表达,在四川黄籽中只在叶片和胚中表达。DFR基因在四川黄籽种皮中不表达,导致种皮中花色素和原花色素不能合成,从而种皮透明,形成黄籽,因此DFR基因是油菜种皮颜色形成途径中一个关键基因。本研究为利用该基因与种子、种皮特异启动子构建反义表达载体或RNAi载体,阐明油菜种皮颜色形成的分子机理和创造黄籽油菜新种质奠定了基础。
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页码:1 / 7
页数:7
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