绿色荧光蛋白的改进

被引：4

作者：

杨广

Wang-Pruski Gefu

尤民生

机构：

[1] 福建农林大学植保学院

[2] Nova Scotia Agricultural College

[3] Truro

[4] NS

[5] BN E

[6] Canada

[7] 福建农林大学植保学院福州

[8] 福州

来源：

农业生物技术学报 | 2002年 / 04期

关键词：

绿色荧光蛋白; 基因优化; 氨基酸替换;

D O I：

暂无

中图分类号：

Q51 [蛋白质];

学科分类号：

摘要：

绿色荧光蛋白(green fluorescent protein,GFP)是细胞生物学和分子生物学研究的一个重要的工具,已得到广泛的应用。为了进一步提高GFP的利用,GFP在几个方面得到了改进:①基因优化,包括去除隐蔽性内含子,在隐蔽内含子的结合位点引入植物内含子,合成密码子用于优化新基因,对GFP进行环状序列改变。②通过氨基酸替换提高GFP脱辅基蛋白在高温,如37℃条件下的正确折叠,以及改变GFP光谱特性。此外,把GFP和定位序列融合,能够提高它的亚细胞定位,从而减少毒性和增加荧光。

引用

页码：397 / 403

页数：7

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