反相高效液相色谱法测定怀牛膝中羟基促蜕皮甾酮的含量

被引：13

作者：

马英

车镇涛

毕开顺

王玺

黄伟

机构：

[1] 沈阳药科大学!沈阳,香港中文大学中医学院!香港,沈阳药科大学!沈阳,沈阳药科大学!沈阳,沈阳药科大学!沈阳

来源：

药学学报 | 2000年 / 04期

关键词：

怀牛膝; 羟基促蜕皮甾酮; 反相高效液相色谱法;

D O I：

10.16438/j.0513-4870.2000.04.019

中图分类号：

R284 [中药化学];

学科分类号：

1008 ;

摘要：

AIM: To set up an assay method for the determination of ecdysterone (EDS) in the root of Achyranthes bidentata Bl. by RP HPLC. METHODS: The EDS was separated on a Hypersil ODS column (200 mm×4 6 mm ID, 5 μm), using acetonitrile — water (1 0∶5 4) as the mobile phase and detected at 243 nm. The flow rate was 1 0 mL·min -1 . Phenol was used as an internal standard. RESULTS: The calibration curve was in good linearity over the range of 28 04～280 40 μg (γ=0 9997). The average recovery was 95 2%. CONCLUSION: The method is simple, fast, sensitive and is suitable for quantitative analysis of EDS in the root of Achyranthes bidentata Bl.

引用

页码：313 / 315

页数：3

共 4 条

[1] 牛膝中齐墩果酸薄层扫描测定方法的研究 [J].