二次回归正交设计在微型月季组织培养中的应用

Quadratic regressive factorial experiment was used to optimize concentrations of 6BA and NAA added in MS midum for shoot multiplication of miniature rose （Rosa cv.Sonnen Kind）.The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68～2.75 mg·L -1 and 0.07～0.54 mg·L -1, respectively, and each 10 explants （stems with one auxiliary bud） cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.