粉虱传双生病毒的TAS-ELISA及PCR快速检测

被引：68

作者：

谢艳

张仲凯

李正和

丁铭

周雪平

机构：

[1] 浙江大学生物技术研究所

[2] 云南省农科院生物技术研究所

来源：

植物病理学报 | 2002年 / 02期

关键词：

粉虱传双生病毒; 三抗体夹心ELISA; PCR; 检测;

D O I：

10.13926/j.cnki.apps.2002.02.015

中图分类号：

S432.41 [];

学科分类号：

摘要：

利用粉虱传双生病毒 (WTGs)多克隆抗体及单克隆抗体 ,建立了三抗体夹心 EL ISA (TAS-EL ISA)检测 WTGs的方法 ,并发现了单克隆抗体 SCR18可广泛用于我国 WTGs的检测。利用根据WTGs基因组上基因间隔区及外壳蛋白基因保守序列设计的引物 ,建立了 PCR特异检测 WTGs的方法。对田间病样的 TAS- EL ISA和 PCR检测表明 ,粉虱传双生病毒在云南省烟草、番茄和南瓜上均存在 ,2种方法的测定结果是一致的。

引用

页码：182 / 186

页数：5

共 7 条

[1]

Possible emergence of new geminiviruses by frequent recombination. PADIDAM M,SAWYER S,FAUQUET C M. Journal of Virology . 1999

[2]

Plant pathology-geminiviruses emerge as serious crop threat. MOFFAT A S. Science . 1999

[3]

Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (Begomoviruses). HARRISON B D,ROBINSON D J. Annual Review of Phytopathology . 1999

[4]

Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra. ZHOU X P,LIU Y L,ROBINSON D J,et al. Journal of General Virology . 1998

[5]

Role of a novel type of double infection in the geminivirus-induced epidemic of severe cassava mosaic in Uganda. HARRISON B D,ZHOU X,OTIM-NAPE G W,et al. Annals of Applied Biology . 1997

[6]

Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. ZHOU X P,LIU Y L,CALVERT L,et al. Journal of General Virology . 1997

[7]

Detection and relationships of cotton leaf curl virus and allied whiteflytransmitted geminiviruses occurring in Pakistan. HARRISON B D,LIU Y L,KHALID S,et al. Annals of Applied Biology . 1997

← 1 →