新型快速色谱柱分离头孢类抗生素的比较

被引：2

作者：

曹小吉

黄琳娜

叶学敏

莫卫民

机构：

[1] 浙江工业大学分析测试中心

来源：

理化检验(化学分册) | 2010年 / 46卷 / 12期

关键词：

Halo-C18色谱柱; 头孢菌素; 柱效; 分离度;

D O I：

暂无

中图分类号：

O657.7 [色谱分析];

学科分类号：

摘要：

对多孔壳层Halo-C18色谱柱、Zorbax Eclipse XDB-C18色谱柱、Zorbax SB-C18色谱柱、ACQUIT UPLC BEH-C18色谱柱以及多孔壳层Halo-C18与Zorbax SB-C18的串联色谱柱分离分析头孢菌素类药物进行了比较研究。结果表明:多孔壳层Halo-C18色谱柱的分离效果明显优于Zorbax Eclipse XDB-C18色谱柱和Zorbax SB-C18色谱柱。串联柱的柱效、分离度几乎达到ACQUITUPLC BEH-C18色谱柱的水平,而其柱压却只有ACQUIT UPLC BEH-C18色谱柱的一半。多孔壳层Halo-C18与Zorbax SB-C18串联色谱柱在一定程度上可以取代超高压色谱柱在常规液相色谱仪上实现高通量快速分离。

引用

页码：1418 / 1420+1424 +1424

页数：4

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